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RNA Seq
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0
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9
replies
2.6k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 3.5 years ago by
bernatgel
▴ 150 • written 4.5 years ago by
adeler001
• 0
2
votes
5
replies
3.2k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.0 years ago by
Steve Lianoglou
★ 13k • written 4.0 years ago by
svlachavas
▴ 830
0
votes
3
replies
645
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.0 years ago by
Kevin Blighe
★ 3.9k • written 4.1 years ago by
isabel.pieterse
• 0
3
votes
3
replies
874
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 4.4 years ago by
Leonard Goldstein
▴ 180 • written 4.4 years ago by
116100016
• 0
1
vote
4
replies
890
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 4.4 years ago by
James W. MacDonald
65k • written 4.4 years ago by
abf
▴ 30
0
votes
0
replies
491
views
searching for specific RNA sequences
rna seq
4.4 years ago
adeler001
• 0
0
votes
2
replies
773
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 4.5 years ago by
Leonard Goldstein
▴ 180 • written 4.5 years ago by
116100016
• 0
0
votes
1
reply
548
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 4.6 years ago by
Michael Love
41k • written 4.6 years ago by
adeler001
• 0
0
votes
2
replies
942
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 4.7 years ago by
Michael Love
41k • written 4.7 years ago by
adeler001
• 0
1
vote
5
replies
1.3k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
mahes.muniandy
• 0
5
votes
3
replies
953
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 4.9 years ago by
Peter Langfelder
★ 3.0k • written 4.9 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
1.9k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.0 years ago
Mozart
▴ 30
0
votes
0
replies
1.6k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.2 years ago
lawarde.ankita1
▴ 30
13 results • Page
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Answer: Dominance Index for Shotgun Metagenomics Data
by
Leo Lahti
▴ 40
1. Note that Simpson's dominance index (\lambda) is a different index than inverse Simpson (1/\lambda) or reciprocal Simpson (1-\lambda). T…
Comment: Math expression in shapeCustom legend in EnhancedVolcano
by
pl23
• 0
Thank you very much - that worked perfectly! Word of warning to those who try this: it screws up the other legends because of the markdown …
Answer: PCA plot suggestions
by
Michael Love
41k
For help with interpretation and design of analysis, I recommend working with a local statistician. Due to time restrictions, I can only…
Comment: DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
by
ussarizona
▴ 10
Hi Michael, Yes, I could figure it out what does the error mean. In my matrix I had the first colum GeneID while in my condition I only h…
Answer: Deseq2. decontXcounts not integers. Useful alternative?
by
Michael Love
41k
Another option would be to put the per sample estimated contamination in the design. This is for example how RUV etc help remove spurious D…
Votes
Answer: Dominance Index for Shotgun Metagenomics Data
Answer: Math expression in shapeCustom legend in EnhancedVolcano
Comment: DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
Comment: Deseq2. decontXcounts not integers. Useful alternative?
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
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